SEPARATION OF PLATELET * LIMPHOCYTE AND LARGE MONOCYTE FROM ANTICOAGULANT TREATED BLOOD

摘要

Abstract of the DisclosureThis invention relates to a method for isolatingplatelets, lymphocytes, and monocytes from anticoagulatedblood. The method involves centrifuging at high force asample of uncoagulated blood with a water-insoluble thixo-tropic gel-like material having a specific gravity between1.065-1.077g/cc for a length of time sufficient to cause thegel-like material to form a barrier between the platelets,lymphocytes, monocytes, and plasma fraction of the blood andthe heavier blood cells. Thereafter, the plasma is withdrawnand the platelets, lymphocytes, and monocytes removed fromabove the barrier in a buffer solution. Where desired, theplatelets can be separated from the lymphocytes and monocytesutilizing a similar process but wherein the water-insolublethixotropic gel-like material has a specific gravity of lessthan about 1.055g/cc.