Hatsukohobitaminb12noseiho

摘要

PURPOSE:To prepare vitamin B12 in high yield, by culturing a vitamin B12- producing bacterial strain belonging to Propionibacterium genus in a medium containing fructose as a carbon source. CONSTITUTION:A vitamin B12-producing strain belonging to Propionibacterium genus, e.g. Propionibacterium shermanii IFO 12391, Propionibacterium freudenreichii IFO 12424, etc., is cultured in a medium containing fructose and optionally glucose as carbon sources (e.g. a medium containing hydrolyzed black strap molasses containing fructose and glucose) at about 25-37 deg.C and about 4-8.5 pH under anaerobic condition, and vitamin B12 is separated from the cultured bacterial cells.