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METHOD OF EXPRESSIVE VECTOR PRODUCTION CAPABLE TO GIVE MATURE HUMAN LEUCOCYTIC INTERFERON IN TRANSFORMED STRAIN OF ESCHERICHIA COLI BY MEANS OF EXPRESSION
METHOD OF EXPRESSIVE VECTOR PRODUCTION CAPABLE TO GIVE MATURE HUMAN LEUCOCYTIC INTERFERON IN TRANSFORMED STRAIN OF ESCHERICHIA COLI BY MEANS OF EXPRESSION
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机译:用表达方式转化大肠埃希氏大肠杆菌转化株中成熟的人白细胞干扰素的表达载体生产方法。
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摘要
A method of production of an expressive vector capable in a transformed Escherichia coli strain of producing mature human leukocytic interferon with a partial amino acid sequence Cys-Ala-Trp-Glu-Val-Val-Arg-Ala-Glu-Ile-Met-Arg-Ser with 165 to 166 amino acids and with the amino acid Asp, Glu or Val in position 114 or, if methionine is linked to the N-terminal of the first amino acid of the abovementioned interferon, with 166 to 167 amino acids and with the amino acid Asp, Glu or Val in position 115, based on the fact that a) an E. coli vector is engineered containing an E. coli trp promoter, operator and trp leader of the ribosome binding point, b) a DNA fragment is prepared coding mature human leukocytic interferon, selected from a group consisting of human leukocytic interferon (LeIF) A, B, C, D, F, H, I and J, c) the abovementioned DNA fragment human leukocytic interferon is inserted in the direction of the fibre from the E. coli trp promoter, operator and trp leader of the ribosome binding point, whereby an E. coli expressive vector is acquired able, by expression in E. coli, to mature human leukocytic interferon.
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