Process for the preparation of human insulin, Des-PheB1 human insulin or derivatives thereof from porcine insulin, Des-PheB1 porcine insulin or derivatives thereof

摘要

What is disclosed is a method for making human insulin or modified human insulin from pig insulin or modified pig insulin by reacting the pig insulin starting material at a pH below its isoelectric point with an excess of a threonine ester in the presence of trypsin or a trypsin-like enzyme.