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Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli
Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli
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机译:具有翻译起始-终止-起始密码子构型的质粒,用于在大肠杆菌中表达蛋白质
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摘要
The invention relates to expression plasmids which bring about a considerable increase in expression of proteins, in particular of the thermolabile E.coli enterotoxin subunit B (LT-B). This entails a strong promoter being placed in front of the DNA coding for LT-B so that the so-called Shine-Dalgarno sequence GGGA of the LT-B gene is located six base-pairs (bp) in front of the translation start of the LT-B, and where the stop codon TGA of the LT-A sequence, which precedes in the natural case, is retained.
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