A method for the preparation of human antithrombin III (ATIII) and of pharmaceutical compositions comprising it as well as of suitable host cells and cells for the purpose of human metabolism

摘要

Human antithrombin III (ATIII) which has progressive inhibitory activity and is stimulated by heparin is advantageously obtained by expression after cotransfection of mammalian cells whose dihydrofolate reductase (DHFR) activity is deficient (dhfr-) with a) vectors which contain the ATIII cDNA and b) vectors which harbour the DHFR gene, especially after gene amplification with methotrexate.