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PURIFICATION,CHARACTERISATION AND APPLICATION OF A NEW NEUROTROPHIC FACTOR DERIVED FROM MAMMALIAN BRAIN
PURIFICATION,CHARACTERISATION AND APPLICATION OF A NEW NEUROTROPHIC FACTOR DERIVED FROM MAMMALIAN BRAIN
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机译:哺乳动物脑中新的神经营养因子的纯化,鉴定和应用
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摘要
A novel neuronotrophic factor having a molecular weight of about l4,400 daltons and an isoelectric point of about l0 is prepared by homogenization of mammalian brain tissue, particularly bovine brain tissue, acid precipitation of the homogenate thus produced, dialysis of the resulting supernatant with dialysis membranes having a molecular weight cut-off of between 5 and l0 kilodaltons and chromatographic fractionation upon molecular weight permeation of the dialyzed supernatant thus produced. The neuronotrophically active fractions may be further purified by cation exchange chromatography with a gradient of ammonium acetate buffer. The neuronotrophic factor of the invention is useful in the treatment of various neuropathological conditions.
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