mammalian monoclonal antibodies against endotoxin of gram negative bacteria and their use in therapy, diagnosis and prophylaxis

摘要

Mammalian monoclonal anti-LPS antibodies that react with regions of lipopolysaccharide which are common to the lipopolysaccharide molecules of gram-negative bacteria of different genera are described along with diagnostic and therapeutic uses of the anti-LPS antibodies. The monoclonal anti-LPS antibodies react with gram-negative bacteria across different genera, reacting with regions of the lipid A and/or the core of gram-negative bacterial endotoxin which are shared by virtually all types of gram-negative bacteria. The anti-LPS antibodies protect mice against the lethal effect from gram-negative bacterial infection and neutralize the lethal effect of the endotoxin of gram-negative bacteria in vivo. Also disclosed are immortal antibody-producing cells such as mammalian hybridoma cell lines which generate the monoclonal anti-LPS antibodies. The mammalian monoclonal anti-LPS antibodies are useful in a number of diagnostic and therapeutic applications, including methods for the detection of gram-negative bacteria or endotoxin of gram-negative bacteria, therapy of gram-negative bacterial infection and endotoxin shock in mammals and prophylaxis against bacterial infection in mammals.