HOMOGENEOUS PROCESS FOR THE LUMINESCENT DETERMINATION AND/OR DETECTION OF AN ANALYTE IN A MEDIUM SUSCEPTIBLE OF CONTAININGIT

摘要

Homogeneous process for the detection and/or determination by luminescence of an analyte in a medium susceptible of containing it by evidencing the product obtained by the reaction between the analyte and a corresponding receiver. Said process comprises 1) the addition to said medium of a first reactant comprised of an analyte receiver; 2) the addition of a second reactant comprised of at least one of the elements of the product obtained from the reaction of the analyte with at least one of its receivers, one of the two reactants being coupled with a luminescent compound and the other reactant comprising a heavy atom or patterns containing a heavy atom; 3) the incubation of the resulting medium either after the addition of each reactant or after the addition of both reactants; 4) excitation of the resulting medium; and 5) measuring, in balance or kinetic conditions, the signal emitted by the luminescent compound, said signal being modulated by the heavy atom effect.