Method and reagent for the specific determination of HDL-Cholesterol in serum

摘要

The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement. The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains 0.1 to 10 U/ml. cholesterol esterase 0.05 to 10 U/ml. cholesterol oxidase 20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0) 0.2 to 20 mmole/liter of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate and, separately therefrom, 0.02 to 2% non-ionic polyethylene oxide group-containing detergent or secondary alkane sulphonate, and optionally 0.05 to 2% of an alcohol containing up to 3 carbon atoms.