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METHOD FOR THE IMMERSED CULTURE OF PSEUDOMONAS AERUGINOSA BACTERIAL STRAINS

机译:沉浸式铜绿假单胞菌细菌培养方法

摘要

Method for the immersed culture of Pseudomonas aeruginosa bacterial strains which produce H type antigens, wherein the culture is preferably carried out continuously in a synthetic aqueous medium containing a carbon source, mineral salts and a nitrogen source. As carbon source, a succinate or urea is used. The culture medium must be free of proteins and antigens. A bacterial biomass is obtained of which the physiological state remains constant throughout the culture and does not contain undesirable impurities such as degradation products from the medium.
机译:产生H型抗原的铜绿假单胞菌细菌菌株的浸没培养方法,其中培养优选在含有碳源,无机盐和氮源的合成水介质中连续进行。作为碳源,使用琥珀酸酯或尿素。培养基必须不含蛋白质和抗原。获得细菌生物质,其在整个培养过程中的生理状态保持恒定,并且不包含不希望的杂质,例如来自培养基的降解产物。

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