Preparation of a library of peptidic antigenic determinants, new peptides built from or containing these determinants and use thereof, namely for diagnosis

摘要

Method for preparing a library of antigenic peptide continuous determinants of a protein or a plurality of proteins expressed by an organism or a microorganism and corresponding to its genome or to a portion of its genome, method whereby said genome or part of the genome is fragmented randomly into a multitude of small fragments which are then expressed in an expression system, for example a yeast, having no homology with said genome by means an appropriate expression vector and the totality or major part of expression products is riddled with monoclonal or non-monoclonal antibodies. The method is applicable particularly to the forming of a library of peptides carrying the antigenic determinants of a virus such as HIV, and in particular HIV1.