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Method of immunologically assaying human thrombin-antithrombin III complex, assay reagent and kit therefor
Method of immunologically assaying human thrombin-antithrombin III complex, assay reagent and kit therefor
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机译:免疫测定人凝血酶-抗凝血酶III复合物的方法,测定试剂和试剂盒
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摘要
A method of immunologically assaying a human thrombin-antithrombin III complex in a human assay sample by using a first antibody composed of an insoluble carrier and an anti-human thrombin polyclonal antibody fixed to the insoluble carrier and a second antibody composed of an anti-human antithrombin III antibody and a labelling substance bound thereto; wherein(1) the anti-human thrombin polyclonal antibody has(a) a dissociation constant with respect to human thrombin of 2.5 x 10⁻⁹M to 3.5 x 10⁻⁹M,(b) a dissociation constant with respect to human prothrombin of 4.5 x 10⁻⁷M to 5.5 x 10⁻⁷M,(c) a dissociation constant with respect to the human thrombin-antithrombin III complex of 0.5 x 10⁻⁹M to 1.5 x 10⁻⁹M, and(d) a cross-reactivity with human prothrombin of not more than 0.05 %, and(2) the anti-human thrombin polyclonal antibody is bound to the insoluble carrier at a rate of 2 to 25 % based on the saturated amount of the polyclonal antibody bound to the insoluble carrier, and a kit therefor.展开▼