The production method of heidelo Corzine caused by biotransformation

摘要

A process for preparing prednisolone from hydrocortisone comprises: (a) pre-culturing arthrobactor simplex at 30 deg.C, pH 7.3 and 150-180rpm in the medium to obtain the starter; (b) innoculating the starter to the culture medium contg. 0-0.2gr. glucose, 0.1-1.0gr. casamino acid, 0.05-0.5gr. KH2PO4, 0.05-0.5gr. K2HPO4, 0.001-0.01gr. Mgcl2, 0.05gr. tween 80 and 100ml D.W., and culturing at 30-37 deg.C, pH 6-9 and 150-180rpm for 4hrs.; (c) adding hydrocortisone powder into the culture broth and culturing at the same condition for 28hrs to obtain the final product. The prednisolone is useful as an antiphlogistic.