VECTOR PLASMIDE DNA FOR CLONING FOREIGN GENES IN ESCHERICHIA COLI AND BACILLUS SPP GENES, AND METHOD OF ITS CONSTRUCTION

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to genetic engineering and u0441u0432u00a0u0437u0430u043du043e to u043fu043eu043bu0443u0447u0435u043du0438u00a0 new u0431u0438u0440u0435u0433u043eu0442u0438u043au043eu043du043du043eu0433u043e vector. designed u0431u0438u0440u0435u043fu043bu0438u043au043eu043du043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430, u043fu0440u0435u0434u043du0430u0437u043du0430u0447u0435u043du043du0430u00a0 u0434u043bu00a0 u043au043bu043eu043du0438u0440u043eu0432u0430u043du0438u00a0 fragments of alien dna in cells of escherichia coli and 5 different types of bacillus spp.u0434u043bu00a0 this u043fu043bu0430u0437u043cu0438u0434u0443 figure 19 subjected to partial hydrolysis u044du043du0434u043eu043au0443u043au043bu0435u0430u0437u043eu0439 u043du0440u043011, u043fu043bu0430u0437u043cu0438u0434u0443 far 16 u0440u0430u0441u0449u0435u043fu043bu00a0u044eu0442 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u043eu0439 EcoR i, sticky ends of fragments add d. on going through the u043au043bu0435u043du043eu0432u0430 dna adp ribose polymerase 1, then u043bu0438u0433u0438u0440u0443u044eu0442 with each other, a mixture of molecules to transform strain of e.coli, vm 83 and selected clones with u0444u0435u043du043eu0442u0438u043fu043eu043c tc, Amp 1, LacZ containing u0431u0438u0440u0435u043fu043bu0438u043au043eu043du043du0443u044e u043fu043bu0430u0437u043cu0438u0434u0443, u0442u0440u0430u043du0441u0444u043eu0440u043cu0430u043du0442u043eu0432 u0432u044bu0434u0435u043bu00a0u044eu0442 u043fu043bu0430u0437u043cu0438u0434u043du0443u044e dna u043fu0440u043eu0432u043eu0434u00a0u0442 u0440u0435u0441u0442u0440u0438u043au0446u0438u043eu043d - u043du044bu043d analysis and all plasmids of minimum size. one of the selected plasmids is like. m. 4.2 Ttn.o., which indicates pOLYA 15. it consists of rice t9 plasmid size of 1.7 u0442.u043f.u043e.limited anr c fragments of size nto 404 and 34, and a sequence of forces v gene lac z 7 with u043fu043eu043bu0438u043bu0438u043du043au0435u0440u043du043eu0439 sequence, including sites u0434u043bu00a0 10 d u0441u0442u0440u0438u043au0442u0430u0437 (EcoRI, sad, Kpnl, Sma. i, bam h i, Xba i and sal i, PstI, SphI, Hinl iii).the second component of the hybrid plasmids u00a0u0432u043bu00a0u0435u0442u0441u00a0 EcoR i fragment of the plasmid, which 16 wherein forces v of the plasmids and gene re u0437u0438u0441u0442u0435u043du0442u043du043eu0441u0442u0438 to u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0443 tc time mayor, 3.1 u0442.u043f.u043e. u043fu043eu043bu0443u0447u0435u043du043du0430u00a0 u0433u0438u0431u0440u0438u0434u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 stable u043du0430u0441u043bu0435u0434u0443u0435u0442u0441u00a0 both in cells and in cells Eecherichia coli, bacillus spp., 2 boeing. s - lu. i (l with s of gs with the ma