STRAIN OF HYBRID CULTIVATED CELLS OF ANIMALS MUS MUSCULUS USED FOR OBTAINING MONOCLONAL ANTIBODIES TO GROUP SPESIFICANTIGEN N OF MNS SYSTEM ERYTHROCYTES OF MAN

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology and can be used u0434u043bu00a0 u0432u044bu00a0u0432u043bu0435u043du0438u00a0 u0433u0440u0443u043fu043fu043eu0441u043fu0435u0446u0438u0444u0438u0447u0435u0441u043au043eu0433u043e n antigen in u044du0440u0438u0442u0440u043eu0446u0438u0442u0430u0445 rights. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 reduction process u043fu043eu043bu0443u0447u0435u043du0438u00a0 monoclonal antibodies by u0441u043eu0437u0434u0430u043du0438u00a0 new strain of hybrid cells of animal mus MUSCULUS, u0441u0435u043au0440u0435u0442u0438u0440u0443u044eu0449 the monoclonal antibodies, specifically u0432u044bu00a0u0432u043bu00a0u044eu0449u0438u0435 n antigen in u044du0440u0438u0442u0440u043eu0446u0438u0442u0430u0445 rights.the strain produced u0441u043bu0438u00a0u043du0438u0435u043c mouse myeloma ra - u044563 -. 8.653 with spleen cells from immunized mice BALB / c, u044du0440u0438u0442u0440u043eu0446u0438u0442u0430u043cu0438 rights u0444u0435u043du043eu0442u0438u043fu0430 on under the influence of u043fu043eu043bu0438u044du0442u0438u043bu0435u043du0433u043bu0438u043au043eu043bu00a0 u043c.u0432.3000 and further cultured u0438u00a0 in the selective environment gat. the messenger substances, with which cells and u0441u043fu043bu0435u043du043eu0446u0438u0442u043eu0432 1: 3. the cells u043fu0430u0441u0441u0438u0432u0438u0440u0443u044eu0442 every 2 - 3 u0434u043du00a0.u0441u0435u043au0440u0435u0442u0438u0440u0443u0435u043cu044bu0435 u043cu043eu043du0430u0442 on the third day to reach the u0442u0438u0442u0440u0430 1: 64 - 1: 256 (u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 fluid) and 1: 1 - 1: 2800 (u0430u0441u0446u0438u0442u043du043eu0439 fluid). the strain is deposited with the number u0432u0441u043au043a (p) n 255u0434. u043cu043eu043du0430u0442 can be used u0434u043bu00a0 u0432u044bu00a0u0432u043bu0435u043du0438u00a0 n antigen in u044du0440u0438u0442u0440u043eu0446u0438u0442u0430u0445 and other human cells. 1, table 2).