amylase inhibitor, methods for its production from wheat and its use as active substance in an agent for the fractional quantitative analysis of - amylase - iso zymen.the wheat amylase inhibitor obtained is soluble in water or dilute saline solutions and in methanol, ethanol, acetone, chloroform and hexane insoluble; he has a uv absorptionsmaximum at 279 nm, with the e 79q601% in water 12.8 (1 cm), he has a molecular weight v on 23 and 23800, determined by ultrazentrifugierung, or of 24000.determined by gelfiltrierung; it provides a single band with a elektrophoretischen mobility of 0.53 in the polyacrylamidgel - electrophoresis according to the method described by david et al. after the reduction of 2 - mercapto ethanol in 6 m urea or the subsequent carboxymethylierung with monojodessigsu00e4ure returns in the electrophoresis according to the method of davis et al on ca rwendung of 6 m urea containing medium, a single band with a elektrophoretischen mobiquality of 0.60; for sds electrophoresis polyacryl amidgel - according to the procedure of oath et al provide at a molecular weight (mg) of 13000 corresponding part of a single band; in the analysis after the procedure of iwanaga et al, n - terminal serine.the existence of two identical subunits display; he has a protein nature with a specific amino acid composition and returns.
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