SINGLE DYE REPLACEMENT FOR ROMANOWSKY PLURAL DYE VARIABLE COMPOSITIONS IN HUMAN BIOPSY SPECIMEN CONSTITUENT IDENTIFICATIONS

摘要

The present invention provides an advance in the art of cytology of blood, bone marrow and like tissue cell studies disclosing unique qualities and abilities of an unusual single cationic sulphur containing azo textile dye which in dilute aqueous solution stains identifying components present in the fixed human biopsy specimens after said specimens have been stained in a variety of different but reproducibly identifying metachromatic colors and patterns of colors having unusual color stain stability over extended time periods. Basic blue 41 makes possible, not only differentiation and identification of all the previously known blood cells identified by the Romanowsky-type dyes collectively in present day use but has been more recently established to differentiate and identify megakaryocytes, sub-populations of lymphocytes, including: B-cells, T-helper cells, T-suppressor cells, Natural Killer or NK cells, as well as blood parasites characteristic of certain tropical diseases, illustratively, Plasmodium Falciparum and Trypanosomiasis.