DNA sequence comprising the functional part of the sacUh locus of B. subtilis, vectors containing the corresponding sequence, and their use in processes for producing proteins

摘要

The invention relates to a nucleic acid fragment containing a functional nucleotide sequence contained in a 14 kb fragment obtained from the chromosome from a B. subtilis strain having a sacUh mutation, the said sequence being characterised in that it is capable, when it is introduced into B. subtilis - or into another microorganism, capable of exploiting to its advantage the information carried by this fragment - of inducing therein an overproduction of proteins or of conferring on it a Degh phenotype. The invention also relates to a process for stimulating the production of a determined polypeptide by an appropriate microorganism by introducing the abovementioned DNA fragment into the cells of the microorganism whose genome or chromosome has been modified so that it contains a DNA sequence which encodes the said determined polypeptide.