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PROCESS FOR PRODUCING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY,NOVEL SUPEROXIDE DISMUTASE ENZYMES AND NOVEL PHARMACEUTICAL COMPOSITIONS COMPRISING ENZYMES
PROCESS FOR PRODUCING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY,NOVEL SUPEROXIDE DISMUTASE ENZYMES AND NOVEL PHARMACEUTICAL COMPOSITIONS COMPRISING ENZYMES
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机译:具有超氧化物歧化酶活性的酶,新型超氧化物歧化酶和包含该酶的新型药物组合物的生产方法
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摘要
The genes (sod) encoding Bacillus stearothermophilus and Bacillus caldotenax Mn-superoxide dismutase have been cloned in Escherichia coli and their entire nucleotide sequences determined. With the exception of the post-translationally cleaved N-terminal methionine residue, the predicted amino acid sequence of the B. stearothermophilus enzyme (BSMnSOD) exhibits 100 % similarity to the previously determined amino acid sequence. The B. caldoenax enzyme (BCMnSOD) differs by two amino acids from BSMnSOD. Both recombinant MnSOD's were shown to be functionally active in E. coli, both in vitro and in vivo, and were expressed to 47 % of the cells soluble protein by coupling their transcription to the E. coli trp promoter. The pharmaceutical use of these bacterial superoxide dismutases is described.
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机译:已经在大肠杆菌中克隆了编码嗜热脂肪芽孢杆菌和caldotenax锰超氧化物歧化酶的基因(sod),并确定了其全部核苷酸序列。除翻译后切割的N-末端甲硫氨酸残基外,嗜热脂肪芽孢杆菌酶(BSMnSOD)的预测氨基酸序列与先前确定的氨基酸序列具有100%的相似性。 B. caldoenax酶(BCMnSOD)与BSMnSOD有两个氨基酸的差异。已显示两种重组MnSOD在体外和体内均在大肠杆菌中具有功能活性,并且通过将其转录偶联至大肠杆菌trp启动子而表达为47%的细胞可溶性蛋白。描述了这些细菌超氧化物歧化酶的药物用途。
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