Antibiotic F-0769, process for its production, and its use as a growth accelerating and feed efficiency increasing agent and as an antitumour agent

摘要

An antibiotic F-0769 having the following properties:P P (1) Outer appearance: White or light yellow powder,P P(2) Melting point: 245-250 C.,PP(3) Specific rotation: [].sub.D.sup.25 =-37.5 (c=1, methanol), P P(4) Solubility in solvents: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate and benzene; and insoluble in water and hexane,PP(5) Elementary analysis (found %): C:57.53, H:7.36, O:21.17, N:12.97,PP(6) Ultraviolet absorption spectrum (as measured in methanol: As shown in FIG. 1, max(E.sub. 1 cm.sup.1%)=213 nm (466), 286 nm (200)PP(7) Infrared absorption spectrum (as measured by KBr method): As shown in FIG. 2, PP(8) Nuclear magnetic resonance spectra (as measured in heavy chloroform): .sup.1 H-NMR spectrum is as shown in FIG. 3, and .sup. 13 C-NMR spectrum is as shown in FIG. 4,PP(9) Distinction among basicity, acidity and neutrality: Neutral substance,P P(10) Amino acid analysis: As a result of the hydrolysis with 6N hydrochloric acid at 110 C. for 18 hours, amino acids i.e. threonine, valine and leucine were detected,PP(11) Color reactions: Positive in the iodine reaction and in the potassium permanganate reaction; and negative in the ninhydrin reaction and in the ferric chloride reaction,PP(12) Thin layer chromatography (by means of silica gel, Art, 5715, manufactured by Merck Co.):P P______________________________________ PPSolvent system Rf value PP______________________________________ PPEthyl acetate 0.16 PPEthyl acetate- methanol (5:1) PP 0.33 PPChloroform- methanol (10:1) PP 0.68 PPEthyl acetate- acetone (1:1) PP 0.31 PPAcetone-benzene (5:1) PP 0.40 PP ______________________________________ PPP P(13) Molecular weight (as measured by Rast method): About 745