Method for micromanipulating cells by moving cell-containing vessel on stage of inverted microscope while pricking cells with tip of stylus

摘要

An inverted-design microscope provided with a micromanipulator so as to be movable along the optical axis in interlocking with the movement of the objective lens for focusing pricking in order that the micromanipulator may be operated minutely and simply on an object to be observed without preventing the illuminating system from being attached, detached, replaced or switched. The micromanipulator is so formed as to be able to prick the object along the optical axis of the objective lens in such a manner that the tip part of a stylus of the micromanipulator is always arranged slightly above a focus position of the objective lens.