Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins

摘要

Disclosed is a method for identifying and isolating gene sequences coding for polypeptide sequences which result in specific functional activities or phenotypes, which may then be synthesized and assembled to form new proteins. The assembled protein may contain only amino acid sequences essential for specific functions, have a new quaternary structure resulting from addition of an amino acid sequence with oligomeric activity, or exhibit multiple activities. Also disclosed is a method for identifying, analyzing and synthesizing amino acid sequences with oligomeric activity which may be used to enhance or add a new activity to a protein.PPIn one embodiment, the piece with oligomeric activity is fused onto a polypeptide with one or more additional activities. In another embodiment, this polypeptide sequence is hybridized with a second polypeptide consisting only of the sequence with oligomeric activity. In a variation of this second method, the first polypeptide may naturally contain a sequence with oligomeric activity and the second polypeptide sequence may be sPPThe Government has rights in this invention pursuant to Grant Number NIH-2-R01-GM23562- 06 awarded by the U.S. Department of Health and Human Services.