PURPOSE:To obtain fats and oils rich in gamma-linolenic ester content, by subjecting a plant belonging to the genus Oenothera to give callus and subjecting it to tissue culture in an organic medium containing a plant hormone. CONSTITUTION:Murashige-Skoog medium is blended with 1ppm 2,4- dichlorophenoxybutyric acid (2,4-D) as auxin and 0.1ppm kinetin as cytokinin. Agar powder in an amount to give 0.9wt% solution is added to the medium to give an agar solution, which is sterilized at 120 deg.C for 15min. On the other hand sterily germinated evening primrose is collected three days after the germination, stem part having 1-5mm of evening primrose is cut into 5mm lengths and allowed to stand in solid medium. The cut stem part is kept in a dark place at 25 deg.C and cultivated for 2-3 weeks. When the steam is cultivated for three weeks, a white callus is developed. It is similarly transplanted to the medium and multiplied.
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