BIOACTIVE PROTEIN IN PHYTOLACCA AMERICANA L.

摘要

NEW MATERIAL:A bioactive protein specified by an amino acid sequence represented by the formula. USE:A preventive against plant virus infection, an antiviral agent and a protein synthesis inhibitor. PREPARATION:For example, seeds of Phytolacca americana L. are crushed using a mixer and subsequently washed with petroleum ether for removal of oily substances and deionized water is added to the resultant defatted powder. The obtained liquid is adjusted to pH 4.0 using hydrochloric acid, stirred for extraction of proteins and subjected to centrifugal separation for collection of an extracted solution. To the resultant extracted solution, ammonium nitrate is added until the solution is saturated in order to precipitate the proteins. The precipitated proteins are separated, dissolved in a small portion of water, dialyzed using a trishydrochloric acid buffer solution (pH8.5) and subjected to the anion-exchange column chromatography for collection of non-adsorbed fractions. The collected fractions are subjected to the cation-exchange column chromatography and then eluted by a linear concentration slope of 0-0.3M common salt, thus obtaining the objective bioactive protein of the formula.