POROUS REGENERATED CELLULOSE MEMBRANE FOR REMOVING MYCOPLASMA AND REMOVAL OF MYCOPLASMA

摘要

PURPOSE:To obtain the title porous regenerated cellulose membrane having high removal ratio of mycoplasma, having specific average pore diameter range by water filtration speed method, specific membrane thickness and pore structure capable of being approximated by multi-layer structure membrane substantially having multi-stage filtration function. CONSTITUTION:The above-mentioned porous regenerated cellulose membrane for removing mycoplasma has 30-100nm average pore diameter 2rf by water filtration speed method and =20mum membrane thickness (d) in a range shown by the formula. The membrane has pore structure capable of being approximated by multi-layer structure membrane substantially having multi-stage filtration function. By using the membrane, mycoplasma can be removed from animal plasma, serum, an aqueous solution of salt or an aqueous solution containing protein or amino acid. In the removal, mycoplasma can be removed in high removal ratio such as =99.999%. In the case of a solution containing protein, permeability of protein is =80% and recovery ratio can be increased =90% by filtration followed by washing with a buffer solution, etc., and filtration.