ANTITUMOR AGENT CONTAINING ANTITUMOR POLYSACCHARIDE ORIGINATED FROM POLYPORUS CONFLUENS AS ACTIVE COMPONENT AND PRODUCTION THEREOF

摘要

PURPOSE:To obtain an antitumor agent effective even to an animal having histocompatibility antigen H-2d by culturing Polyporus confluens in a liquid medium, subjecting the culture liquid to hot-extraction, sterilization and alcohol treatment and using the obtained purified polysaccharide as an active component. CONSTITUTION:Culture liquid produced by culturing Polyporus confluens in a liquid medium is extracted at 100 deg.C for 4hr, sterilized and treated with an alcohol. The precipitate is washed with an alcohol and freeze-dried to obtain a polysaccharide [beta-(1 3)-D-glucan having N-PS: beta-(1 6) branch]. The polysaccharide is oxidized with periodic acid and separated into a supernatant fraction (1) and a gel fraction (2). The supernatant fraction (1) is reduced with NaBH4 to obtain a polysaccharide (N-PS.polyol). The gel fraction (2) is further oxidized with periodic acid and separated into a supernatant fraction (1') and a gel fraction (2'). The gel fraction (2') is reduced with NaBH4 to obtain a polysaccharide (N-PS.polyol gel).