NOVEL ENZYMATIC MEASUREMENT OF D-3-HYDROXYBUTYRIC ACID AND ACETOACETIC ACID IN HUMORS, URINE AND MEASUREMENT REAGENT FOR IT

摘要

PURPOSE:To determine accurately D-3-hydroxybutyric acid or acetoacetic acid without pretreating a test specimen, by adding a lactic dehydrogenase inhibitor to a measurement system. CONSTITUTION:In determine D-3-hydroxybutyric acid by converting D-3-hydroxybutyric acid in the presence of NAD and 3-hydroxybutyric dehydrogenase into acetoacetic acid, measuring change in quantity of NADH occurring during the conversion, a lactic dehydrogenase inhibitor is added to a measurement system. Oxamic acid, and an SH inhibitor(iodoacetamide, mercury hydroxybenzoate, trivalent arsenic compound, etc.) are used as the lactic dehydrogenase inhibitor, and oxamic acid is preferable.