PROCESS FOR INVESTIGATING AN ACELLULAR BIOLOGICAL FLUID FOR CELLULAR ONCOGENIC TRANSCRIPTS OR FRAGMENTS THEREOF

摘要

In a process for investigating an acellular biological fluid for the presence of cellular oncogenic transcripts or fragments thereof to test for malignancy, a) the RNA is concentrated or separated from the whole of the acellular biological fluid in the permanent presence of an effective RNase inhibitor, the RNA is denatured and in this form is either immobilized on a solid carrier or left free in solution; b) the RNA is placed in contact with labelled oncogenic DNA samples to hybridize the latter with the RNA, if a complementary sequence is present which eliminates the excess, non-specifically bound marked oncogenic DNA, and c) the product is tested for the presence of labelled DNA.