NOVEL T-5310 AND ITS SALT AND ANTITUMOR AGENT AND IMMUNOSTIMULATING AGENT CONTAINING THE SAME

摘要

NEW MATERIAL:T-5310 (salt) having the following characteristics. Inhibits the multiplication of sarcoma 180 and has an immunostimulating activity. Insoluble in many organic solvents. The molecular weight is 1000 to 50000. The isoelectric point is near 11. Positive on a phenol sulfuric acid reaction, anthrone sulfuric acid reaction, Lowry.Folin reaction and ninhydrin reaction. Elementary analysis; C: 30 to 35%, N: 2 to 4% and H: 5 to 7%. The saccharide content: 40 to 60% and the protein content: =15%. Limulus test is negative. EXAMPLE:An antitumor agent and an immunostimulating agent. USE:The T-5310 is prepared by dissolving TF-310, etc., collected from the culture solution of a bacterium belonging to the genus Fusobacterium, etc., in Ampholine having a pH of 3.5 to 10, treating the solution with a linear gradient mixer and fractionating the treated solution by an isoelectric point electrophoresis employing a column filled with a filler therein in a state having a sucrose density gradient, followed by collecting a fraction having an isoelectric point of 9 to 12.5 to provide the T-5310.