HUMAN ANGIOGENIN MANIFESTATION VECTOR AND PRODUCTION OF HUMAN ANGIOGENIN

摘要

PURPOSE:To produce human angiogenin by transducing a vector containing fragments of a specific promoter and a DNA coding the amino acid sequence of human angiogenin into Escherichia coli. CONSTITUTION:A vector containing (A) fragment of tac promoter or trp promoter, (B) fragment of the replicator Ori of plasmid pUC 18 and (C) a DNA consisting of base sequence coding the amino acid sequence of human angiogenin is split by using a restriction enzyme such as EcoRI, and the resulting product is incorporated with a gene prepared by binding human angiogenin gene with DNA ligase. Thence, the resultant vector is transduced into Escherichia coil followed by screening to obtain a line of transformed bacteria, which is then put to culture in a specified medium, thus producing the objective human angiogenin.