COLLECTION OF INHIBITORY FACTOR OF COAGULATION SYSTEM EXISTING IN AMEBOCYTE LYSATE OF LIMULUS POLYPHEMUS

摘要

PURPOSE:To raise the sensitivity and reactivity of amebocyte lysate to endotoxin, by subjecting amebocyte lysate of Limulus polyphemus to liquid chromatography using a polysaccharide sulfate as an adsorption carrier. CONSTITUTION:Amebocyte lysate of Limulus polyphemus or a solution containing it is subjected to liquid chromatography using a polysaccharide sulfate such as dextran sulfate agarose, agarose sulfate, etc. as an adsorption carrier, factors of coagulation system caused by endotoxin existing in amebocyte lysate and inhibitory factors are adsorbed on it, the factors of coagulation system are mainly desorbed with a salt solution having =0.5 ionic strength, and the inhibitory factors are eluted with a salt solution having =0.5 ionic strength.