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The new medium containing binder, to adsorcao cromatografica, cromatografica affinity column and process for preparing a means of affinity of avidin aperfeicoado
The new medium containing binder, to adsorcao cromatografica, cromatografica affinity column and process for preparing a means of affinity of avidin aperfeicoado
Improved ligand-containing media, a method for preparing the media, and use of the media in the production of peptides, proteins, and the like, by chromatograptuc separation, are provided. More specifically, the media have permanently attached, via a covalent bond to an inert solid substrate, an avidin polypeptide ligand in the dissociated renatured form which reversibly binds to certain molecules such as proteins, peptide, nucleotides, oligonucleotides, and the like, and to other molecules which bind to avidin via biotinylation or by way of their secondary/tertiary micromolecular structures.
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