首页>
外国专利>
USE OF BACTERIAL LIPOPROTEIN AMINO TERMINUS IN FUSION PLASMIDS FOR IN VIVO EXPRESSION OF LIPID MODIFIED POLYPEPTIDES
USE OF BACTERIAL LIPOPROTEIN AMINO TERMINUS IN FUSION PLASMIDS FOR IN VIVO EXPRESSION OF LIPID MODIFIED POLYPEPTIDES
展开▼
机译:融合脂中细菌脂蛋白氨基末端在脂质修饰多肽体内表达中的应用
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to a fusion plasmid for in vivo synthesis of a lipid modified polypeptide. The plasmid comprises a first DNA sequence encoding a lipoprotein signal peptide, preferably a bacterial lipoprotein signal peptide, and at least the first amino acid of a mature lipoprotein, preferably cysteine. In the DNA sequence, the preferred cysteine codon may be followed by codons for a few amino acids forming a .beta.-turn structure. These amino acids may also include a specific exogenous protease recognition cleavage site. A second DNA sequence encoding the desired polypeptide can be inserted into the fusion plasmid to produce the desired lipid modified polypeptide.
展开▼