NOVEL PROCEDURE FOR OBTAINING PROTEINS STARTING FROM AN EXTRACT OF NON-COLLAGENOUS PROTEINS FROM BONY MATERIAL AND ITS USE FOR THE PREPARATION OF A NOVEL PROTEIN INHIBITING THE PROLIFERATION OF OSTEOBLASTS

摘要

The invention relates to a procedure for obtaining a protein purified to a satisfactory homogeneous state, starting from an extract of non-collagenous proteins of bone matrix. Another subject of the invention is a device for the implementation of the procedure of the invention, a protein inhibiting the proliferation of osteoblasts likely to be obtained by the above-mentioned procedure. The procedure described in the present invention is characterized in that it comprises the following steps: the extract of non-collagenous proteins, solubilized beforehand in a solvent, is subjected to a preparative chromatography in the liquid phase on a column of gel with the aid of an elution buffer and an electric field, under conditions such that the desired protein is eluted, and is found, during or at the end of the chromatography, to be present in solution in the elution buffer, alone and free from contaminants, in at least one of the fractions collected at the exit of the column, the fraction containing the desired protein in solution in the elution buffer is recovered, the elution buffer is removed and the desired protein is recovered which, if necessary, is subjected to a renaturation step and/or a reactivation step.