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Expression plasmids for improved production of heterologous protein in bacteria
Expression plasmids for improved production of heterologous protein in bacteria
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机译:表达质粒可改善细菌中异源蛋白质的产生
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摘要
Promoter-ribosome binding site (rbs) expression ele-' ments of general utility for high level heterologous gene expression; plasmids carrying said promoter-rbs expression elements and encoding genetic information for direct high level expression in bacteria of heterologous proteins, especially plasmids carrying a gene coding for prorennin or mammalian growth hormones; methods for their construction, including the use of synthetic linkers to provide desirable functional properties thereto; recombinant microorganisms comprising said plasmids; expression of said bacterial produced heterologous proteins by said recombinant microorganisms; and demonstration of the activities of the thus-produced proteins.
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