Quantitative determn. of adenosine-tri:phosphate - by a bio-luminescent reaction capable of determining ATP in somatic and/or microbial cells

摘要

A process for the quantitative determination of adenosine triphosphate, ATP, in biological material contg. living somatic cells and/or living microbial cells comprises either (i) determining both somatic and microbial cells by (a) obtaining a sample of the biological material; (b) adding at least one detergent to extract the ATP from the somatic cells; (c) adding a reagent for bioluminescent reaction, before, during or after the step (b) extn., which is capable of quantifying the luminescence produced by the non-microbial cells; (d) adding at least one lipid to inhibit inactivation of the bioluminescent reaction and/or the ATP hydrolysis reaction of the detergent; (e) adding at least one enzyme which catalyses hydrolytic cleavage to the mixt. to degrade the ATP form the non-microbial cells; (f) adding a mixt. contg. at least one bactericide to liberate the ATP in the microbial cells and at least one substance which inhibits the activity of the enzyme from (e) but which does not affect the bioluminescent reaction; and (g) quantifying the microbial cells by the bioluminescence produced; or (ii) determining only the microbial cells by carrying out no determination of bioluminescence according to step (c), and then carrying out steps (d), (e), (f) and (g) as above. USE/ADVANTAGE - The process enables ATP to be determined in biological materials such as urine, blood, milk, fruit juices, beer, wine, meat extracts, clear slurry, water, cell cultures, soil samples, foodstuffs and textiles. Mixts. contg. living microorganisms can be analysed even in the presence of living somatic cells.