process for the production of biologically active substances.

摘要

A method of producing biologically active substances using a hybrid cell line obtained by fusing (1) a human tumor cell line which does not grow in a medium containing aminopterin or/and azaserine or cell line derived therefrom with (2) a human non-tumor macrophage. The human tumor macrophage cell line may be either a thymidine kinase-deficient cell line of U-937 or a thymidine kinase-deficient cell line of THP-1, the non-tumor macrophage may be alveolar macrophage, splenic macrophage, peripheral blood monocyte, peritoneal macrophage, hepatic macrophage, placental macrophage, or thymic macrophage, and the biologically active substance produced is a tumoricidal substance or interleukin-1.