The bacterium producing thermostable alpha -amylases of this invention is an anaerobic bacterium belonging to Clostridium. The thermostable alpha -amylases of this invention is novel alpha -amylases which are excellent in thermostability and acid resistance and have a slight calcium requirement. Said alpha -amylases are obtained by culturing the aforesaid bacteria and collecting the alpha -amylases from the culture. When the aforesaid alpha -amylase is used, sugar production process can be greatly rationalized.
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