Mol. wt. determn. from gel permeation chromatograms - calibrated by specimens with known values using nonlinear regression

摘要

Mol.wt. of macro-molecular polymers is determined by a gel permeation chromatographic unit of known type having a column filled with sepn. medium, e.g. polystyrol or kieselgel. Specimen concn. is recorded in relation to elution vol. Chromatograms are derived for a number of specimens with known mol.wt. and used in conjunction with a non-linear regression method in matching unknown parameters to a calibration relation between measured elution vols. and the corresp. mol.wt. The calibration relation provided in the column, to be electronically processed by a computer is based on measured volumes and mathematical constants. ADVANTAGE - Precise method involves min. calculation and determine mol.wts. over entire sepn. range of chromatographic amt. mol.wts.