RAPID METHOD OF DETECTING AND / OR IDENTIFYING ONLY ONE BASED ON A NUCLEIC ACID SEQUENCE, AND ITS APPLICATIONS

摘要

This method is characterized in that: (1) the sequence on which the base to be identified is hybridized with a nucleotide of sufficient length; / P P (2) begins the synthesis of the complementary strand of the hybrid obtained in 1, - said nucleotide serving as a primer -, in the presence of: - a polymerase with no 3 '5' exonuclease action and - at least one modified nucleotide base; / P (3) the blocking nucleotide base incorporated by any suitable means is detected. / P P Application: diagnosis of genetic diseases.