Kit for obtaining a digoxin concentrate from a serum sample

摘要

Serum is passed through a column containing silica gel alkylated with lower alkyl groups. The column is then washed with dilute acid and water and the digoxin eluted with aqueous methanol at a volume less than about the volume of the serum to provide a digoxin concentrate that may be used in assay determinations. The invention also includes a kit comprising in a packaged combination for use in an assay for digoxin (a) a column containing silica gel alkylated with alkyl groups containing 1 to 2 carbon atoms and (b) an aqueous eluent solution which is 50%-70% by volume organic solvent. The organic solvent comprises from 1-4 carbon atoms and from 1-3 heteroatoms selected from the group consisting of oxygen and nitrogen.