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Fluorescent stokes shift probes for polynucleotide hybridization
Fluorescent stokes shift probes for polynucleotide hybridization
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机译:用于多核苷酸杂交的荧光斯托克斯位移探针
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摘要
Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non- radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.
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