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Metabolic pathway engineering to increase production of ascorbic acid intermediates
Metabolic pathway engineering to increase production of ascorbic acid intermediates
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机译:代谢途径工程可提高抗坏血酸中间体的产量
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摘要
In recombinant microorganisms which were rendered capable of converting 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG) by transfer of genetic material, the secondary metabolites and metabolic pathways leading to the metabolic diversion of 2-KLG and 2,5- DKG were determined, and the diversion of 2-KLG to L-iodonic acid (IA) or of 2,5-DKG to 5-keto-D-gluconate (5-KDH) was blocked.
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