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Genetic marker for superior milk products in dairy cattle

机译:奶牛优质奶制品的遗传标记

摘要

An assay for a genetic marker associated with increased milk production is disclosed. Also disclosed are kits for use in connection with the assay and breeding methods that use the assay. The assay centers on finding a genetic marker in a bovine cell (e.g. in the DNA of the cell) . The presence of the marker is confirmed by exposing a gene sequence from the cell to a restriction enzyme so as to yield gene fragments of varying lengths. During a separation step there is a separation of some of the fragments from others (such as by using electrophoresis), and there is then a hybridization of a plurality of probes that contain a portion of bovine prolactin sequence to the separated fragments. The probe is radiolabelled. Then, there is a comparison of the results of the hybridization with the hybridization results for a gene sequence known to either have the marker or not have the marker. The assay appears to be of greatest utility in connection with the Carlin-M Ivanhoe Bell Holstein family.
机译:公开了与增加的牛奶产量相关的遗传标记的测定。还公开了与测定法和使用该测定法的育种方法一起使用的试剂盒。该测定法集中于在牛细胞中(例如在细胞的DNA中)寻找遗传标记。通过将细胞中的基因序列暴露于限制酶,从而产生不同长度的基因片段,可以确认标记的存在。在分离步骤中,将一些片段与其他片段分离(例如通过电泳),然后将包含一部分牛催乳激素序列的多个探针与分离的片段进行杂交。探针被放射性标记。然后,将杂交结果与已知具有标记或不具有标记的基因序列的杂交结果进行比较。该测定法似乎与Carlin-M Ivanhoe Bell Holstein家族最相关。

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