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C-terminal peptide sequencing, activated support and reagent system therefor, and method of producing the activated support
C-terminal peptide sequencing, activated support and reagent system therefor, and method of producing the activated support
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机译:C末端肽测序,活化的支持物及其试剂系统以及产生活化的支持物的方法
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摘要
A method of C-terminal peptide sequencing. The peptide is reacted with an activated support derivatized with a mixed anhydride of isothiocyanic acid and carboxylic or carbonic acid, under basic conditions. The peptidyl thiohydantoin which forms is separated from the solid support and further reacted with a cleaving agent carried on a second solid support, to release a free C-terminal amino acyl thiohydantoin from the peptide. The free thiohydantoin is analyzed to determine the C-terminal peptide residue. The residual peptide can be recycled through the supports for successive C-terminal residue determinations.
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