EPIDEMIC NON-A NON-B HEPATITIS VIRUS ANTIGEN PEPTIDE AND NUCLEIC ACID FRAGMENT CODING THE SAME

摘要

PURPOSE:To enable easy detection of the subject virus by including a specific amino acid sequence. CONSTITUTION:Viral particle originated from the bile of a crab-eating macaque infected with non-A non-B hepatitis virus (KV) is treated with guanidine thiocyanate and a cDNA is synthesized by using the obtained RNA as a template. The cDNA is inserted into a lambdagt11 vector to obtain a cDNA library (A). E.coli infected with a lambda-phage selected from the component A is subjected to replica culture and the obtained replica is blocked to get a replica filter (B). The component B is washed with a phosphate-buffered physiological saline water (PBS) and made to react with the convalescent serum of a KV crab-eating macaque and the serum of a KV patient and then react with an anti-human IgG labeled with an enzyme and an anti-human IgM labeled with an enzyme. The reaction product is screened to obtain a lambda-phage clone. The objective nucleic acid fragment containing nucleic acid sequence of formula II, etc., and coding a KV virus antigen peptide containing the amino acid sequence of formula I, etc., is produced by purifying the component C.