ADDITIVE FOR PEPTIDE SYNTHESIS SOLVENT AND SYNTHESIS OF PEPTIDE USING THE SAME ADDITIVE

摘要

PURPOSE:To obtain an additive for adding to a solvent and peptide synthetic method using the above-mentioned additive in order to prevent elimination of a protective group bound to an amino group in peptide synthesis. CONSTITUTION:The objective additive is at least one kind of additive selected from 1-hydroxybenzotriazole, N-hydroxysuccinic imide and 3-hydroxy-3,4- dihydro-4-oxo-1,2,3-benzotriazine. When an carboxyl group in the first amino acid in which an amino group is bound to a protective group is combined with an amino group in the second amino acid to synthesize a peptide, the additive is added to a Lewis base solvent which dissolves the first amino acid to prevent the amino group from elimination from the protective group. Since side reaction can be prevented without eliminating the protective group even when an amino acid bound to the protective group is allowed to stand in the solvent for a long time, as shown in synthesis of a long-chain peptide or peptide synthesis using a wholly automatic synthetic machine, a peptide having high purity can be obtained.