CLONING VECTOR PLASMID, VECTOR PRIMER DERIVED FROM THE SAME PLASMID AND PREPARATION OF CDNA BANK USING THE SAME PRIMER

摘要

NEW MATERIAL:The title plasmid positioning a restriction enzyme site RE1 from promoter PR1 for mammalian cells toward downstream, promoter PR2 of RNA polymerase, restriction enzyme site RE2, restriction enzyme site RE3, restriction enzyme site RE4, restriction enzyme site RE5 and RNA polymerase promoter PR3, positioning restriction enzyme site RE6 at an arbitrary place of the restriction enzyme site RE1, replication origin OR1 for mammalian cells, replication origin OR2 of single strand phage, replication origin OR3 for Escherichia coil and a selecting marker at an arbitrary position. EXAMPLE:Vector pKA1. USE:Preparation of cDNA bank. PREPARATION:Plasmid pTZ18RP4 obtained from plasmid pTZ18R and plasmid pKAO obtained from plasmid pTZ19U are subjected to a process shown by the formula to give cloning vector pKA1 of example.