PROCESS FOR PRODUCING AND USING NEW GENE MARKED BY T1RB, ENSURING TYLOSIN RESISTANCE AND APPLICABLE WITHIN THE DOMAIN OF STREPTOMYCES AND OTHER MICROORGANISMS

• 1. 生产L-酪氨酸的微生物及利用其生产L-酪氨酸的方法 [P] . 中国专利： CN114072492A . 2022-02-18
• 2. 新型微生物“脱叶链霉菌YJ－118”及使用该菌株生产普伐他丁钠的方法 [P] . 中国专利： CN1126815C . 2003.11.05
• 3. PROCESS FOR PRODUCING AND UTILIZING NEW TLRB-MARKED GENE OF THYLOSIN RESISTANCE UTILIZABLE FOR STREPTOMYCES AND OTHER MICROORGANISMA [P] . HUT44076A . 1988-01-28

  机译： 生产和使用新的TLRB标记的耐链霉菌素基因的链霉菌和其他微生物的基因

• 4. PROCESS FOR THE EXCHANGE OF GENETIC INFORMATION - BY MEANS OF PROTOPLAST FUSION - OF MICROORGANISMS OF THE STREPTOMYCES AND NOCARDIA WITHIN THE GENUS OR BETWEEN THE GENERA [P] . HU189441B . 1986-07-28

  机译： 属或属间链霉菌和MY螨微生物的遗传信息交换-通过原生质体融合的手段。

• 5. TYLOSIN RESISTANCE-CONFERRING GENE,DESIGNATED tlrC, FOR USE IN STREPTOMYCES FRADIAE [P] . IL83254B . 1993-09-22

  机译： 酪氨酸抗性基因，指定为tlrC，用于链霉菌中

• 1. Mutagenesis and Screening of High Avermectin Bla Producing Strains from Streptomyces Avermitilis [J] . Wang Shuyang, Chen Jihong, Liu Jing, 近代物理研究所和兰州重离子加速器实验室年报：英文版 . 2008,第001期
• 2. Studies on the Construction of Genetic Engineering Microorganism to Degrade Organophosphate Residues and to be Marked with Green Fluorescent Protein [C] . Zhu Yaran, Li Chaokun, Qiao Chuanling 第三届“农药与环境安全”国际学术研讨会暨第七届“植物化学保护和全球法律一体化”国际研讨会（3rd I . 2007
• 3. Characterization of putative self-resistance genes in the biosynthetic gene cluster of hygromycin A from Streptomyces hygroscopicus NRRL 2388. [D] . Dhote, Vidya. 2009
• 4. Metabolic regulation in tylosin-producing Streptomyces fradiae: phosphate control of tylosin biosynthesis. [O] . K Vu-Trong, S Bhuwapathanapun, P P Gray 1981
• 5. Metabolic regulation in tylosin-producing Streptomyces fradiae: phosphate control of tylosin biosynthesis. [O] . Vu-Trong, K, Bhuwapathanapun, S, Gray, P P 1981

• 1. Screening of Potato Lines Including Department Genetic amp;National Plant Gene Bank of Iran for Resistance to Early Blight (iAlternaria. solani/i) Using Culture Filtrate Produced by the Fungus [J] . Hamid Reza Mirkarimi ,Ahmad Abasi Moghadam ,Javad Mozafari . 美国植物学期刊（英文） . 2013,第10期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. OXIDATION RESISTANCE OF NANOCRYSTAL ODS ALUMINIDE COATINGS PRODUCED BY PACK ALUMINIZING PROCESS ASSISTED BY BALL PEENING [J] . Z.L. Zhan ,Y.D. He ,W. Gao . 金属学报：英文版 . 2006,第3期
• 4. The genetic architecture of insecticide resistance within a natural population of iDrosophila melanogaster/i [J] . Takahiro Miyo . 遗传学期刊（英文） . 2012,第2期
• 5. 微生物多糖发酵过程的节能减排和清洁生产技术——以微生物多糖结冷胶的发酵生产过程为例 [C] . 詹晓北 ,郑志永 ,朱莉 . 2013中国生物发酵产业年会 . 2013
• 6. 开发适用于链霉菌工程改造的新方法 [A] . 王俊阳 . 2016